Publications

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Recent Publications

  1. MA Winkleman, G Dai. Bioengineered Perfused Human Brain Microvascular Networks Enhance Natural Progenitor Cell Survival, Neurogenesis and Maturation. Science Advances 9 (19), eaaz9499, 2023.
  2. T Bertucci, S Kakarla, MA Winkleman, K Lane, K Stevans, S Lotz, A Grath, D James, S Temple, G Dai. Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells. APL bioengineering 7 (3), 2023. Editor’s featured article.
  3. L Zhang, H Du, X Sun, F Cheng, W Lee, J Li, G Dai, NX Fang, Y Liu. 3D printing of interpenetrating network tough hydrogels with in situ enhancement of adhesiveness. ACS Applied Materials & Interfaces 15 (35), 41892-41905, 2023.
  4. D Kim, A Grath, YW Lu, K Chung, M Winkleman, JJ Schwarz, G Dai. Sox17 mediates adult arterial endothelial adaptation to hemodynamics. Biomaterials, Volume 293, February 2023.
  5. L Zhang, W Lee, X Li, Y Jiang, NX Fang, G Dai, Y Liu. 3D direct printing of mechanical and biocompatible hydrogel meta-structures. Bioactive Materials 10, 48-55, 2022.
  6. MA Winkleman, DY Kim, S Karkala, A Grath, N Silvia, G Dai. Interstitial flow enhances the formation, connectivity, and function of 3D brain microvascular networks generated within a microfludic device. Lab on a Chip 22 (1), 170-192, 2022
  7. Dai G, Feinberg AW, Wan L. Recent Advances in Cellular and Molecular Bioengineering for Buildings and Translation of Biological Systems. Cellular Molecular Bioengineering, 2021, 1-16. CMBE 2022 Most Downloaded Article award.
  8. Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, Dai G, Zou H and Friedel RH. Plexin-B2 facilitates glioblastoma infiltration by modulating cell biomechanics. Communications Biology. 2021;4:145.
  9. Winkelman MA, Koppes AN, Koppes RA and Dai G. Bioengineering the neurovascular niche to study the interaction of neural stem cells and endothelial cells. APL Bioengineering. 2021;5:011507. selected as the featured article.
  10. Soucy JR, Bindas AJ, Brady R, Torregrosa T, Denoncourt CM, Hosic S, Dai G, Koppes AN and Koppes RA. Reconfigurable Microphysiological Systems for Modeling Innervation and Multitissue Interactions. Advanced Biosystems, 2020, 4:9, 2000133.
  11. Silvia N and Dai G. Cerebral Organoids as a Model for Glioblastoma Multiforme. Current Opinion in Biomedical Engineering, 2020:13, 152-159.
  12. Ozturk MS, Lee VK, Zou H, Friedel RH, Intes X and Dai G. High-resolution tomographic analysis of in vitro 3D glioblastoma tumor model under long-term drug treatment. Science Advances. 2020: Vol. 6, no. 10, eaay7513.
  13. Baltazar T, Merola J, Catarino C, Xie CB, Kirkiles-Smith NC, Lee V, Hotta S, Dai G, Xu X, Ferreira FC, Saltzman WM, Pober JS and Karande P. Three Dimensional Bioprinting of a Vascularized and Perfusable Skin Graft Using Human Keratinocytes, Fibroblasts, Pericytes, and Endothelial Cells. Tissue Engineering Part A. 2020, 26 (5-6), 227-238. Featured in cover article.
  14. Xu C, Dai G and Hong Y. Recent advances in high-strength and elastic hydrogels for 3D printing in biomedical applications. Acta Biomaterialia, 2019;95:50-59.
  15. Lee W, Hong Y and Dai G. 3D bioprinting of vascular conduits for pediatric congenital heart repairs. Translational Research. 2019;211:35-45.
  16. Grath A and Dai G. Direct cell reprogramming for tissue engineering and regenerative medicine. Journal of Biological Engineering. 2019;13:14.
  17. Song W, Chiu A, Wang LH, Schwartz RE, Li B, Bouklas N, Bowers DT, An D, Cheong SH, Flanders JA, Pardo Y, Liu Q, Wang X, Lee VK, Dai G and Ma M. Engineering transferrable microvascular meshes for subcutaneous islet transplantation. Nature Communications, 2019;10:4602.
  18. Dorsey TB, Kim D, Grath A, James D and Dai G. Multivalent biomaterial platform to control the distinct arterial venous differentiation of pluripotent stem cells. Biomaterials. 2018;185:1-12.
  19. Kural MH, Dai G, Niklason LE and Gui L. An Ex Vivo Vessel Injury Model to Study Remodeling. Cell Transplantation. 2018;27:1375-1389.
  20. Bertucci TB and Dai G. Biomaterial Engineering for Controlling Pluripotent Stem Cell Fate. Stem Cells International. 2018;2018:9068203.
  21. Niklason L, Dai G. Arterial Venous Differentiation for Vascular Bioengineering. Annu Rev Biomed Eng. 2018 Jun 4;20:431-447.
  22. Xu C, Lee W, Dai G, Hong Y. Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing. ACS Appl Mater Interfaces. 2018 Mar 28;10(12):9969-9979. ACS Editor’s Choice. Top downloaded paper 2018.
  23. Kim D, Lee V, Dorsey TB, Niklason LE, Gui L, Dai G. Neuropilin-1 Mediated Arterial Differentiation of Murine Pluripotent Stem Cells. Stem Cells Dev. 2018 Apr 1;27(7):441-455. Featured in cover article.
  24. Dumont CM, Piselli J, Temple S, Dai G and Thompson DM. Endothelial Cells Exposed to Fluid Shear Stress Support Diffusion Based Maturation of Adult Neural Progenitor Cells. Cell Mol Bioeng. 2018;11:117-130.
  25. Dorsey TB, Grath A, Wang A, Xu C, Hong Y and Dai G. Evaluation of Photochemistry Reaction Kinetics to Pattern Bioactive Proteins on Hydrogels for Biological Applications.  Bioactive Materials. 2018;3:64-73.
  26. Dumont CM, Piselli JM, Kazi N, Bowman E, Li G, Linhardt RJ, Temple S, Dai G, Thompson DM. Factors Released from Endothelial Cells Exposed to Flow Impact Adhesion, Proliferation, and Fate Choice in the Adult Neural Stem Cell Lineage. Stem Cells Dev. 2017, 26(16): 1199-1213.
  27. Lee VK, Dai G. Printing of Three-Dimensional Tissue Analogs for Regenerative Medicine. Ann Biomed Eng. 2017 Jan;45(1):115-131.
  28. Dorsey TB, Grath A, Xu C, Hong Y and Dai G. Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications. J Vis Exp. 2017 Sep 15;(127).
  29. Wu D, Huang RT, Hamanaka RB, Krause MD, Oh MJ, Kuo CH, Nigdelioglu R, Meliton AY, Witt L, Dai G, Civelek M, Prabhakar NR, Fang Y, Mutlu GM. HIF-1α is required for disturbed flow-induced metabolic reprogramming in human and porcine vascular endothelium. Elife. 2017 May 30;6. pii: e25217.
  30. Lu YW, Lowery AM, Sun LY, Singer HA, Dai G, Adam AP, Vincent PA, Schwarz JJ. Endothelial Myocyte Enhancer Factor 2c Inhibits Migration of Smooth Muscle Cells Through Fenestrations in the Internal Elastic Lamina. Arterioscler Thromb Vasc Biol. 2017 May 4. pii: ATVBAHA.117.309180. Featured in cover article.
  31. Zheng Y, Wang S, Xue X, Xu A, Liao W, Deng A, Dai G, Liu AP, Fu J. Notch signaling in regulating angiogenesis in a 3D biomimetic environment. Lab Chip. 2017 May 31;17(11):1948-1959.
  32. Zheng Y, Sun Y, Yu X, Shao Y, Zhang P, Dai G and Fu J. Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic-Cell-Induced Bone Marrow Angiogenesis. Advanced Healthcare Materials. 2016;5:1014-24.
  33. Gao G, Yonezawa T, Hubbell K, Dai G, †Cui X. Inkjet-bioprinted acrylated peptides and PEG hydrogel with human mesenchymal stem cells promote robust bone and cartilage formation with minimal printhead clogging. Biotechnol J. 2015 Jan 8.
  34. Wu C, Huang RT, Kuo CH, Kumar S, Kim CW, Lin YC, Chen YJ, Birukova A, Birukov KG, Dulin N, Civelek M, Lusis AJ, Loyer X, Tedgui A, Dai G, Jo H, Fang Y. Mechano-Sensitive PPAP2B Regulates Endothelial Responses to Athero-Relevant Hemodynamic Forces. Circ Res. 2015 Jul 31;117(4):e41-53.
  35. Gao G, Schilling AF, Hubbell K, Yonezawa T, Truong D, Hong Y, Dai G, Cui X. Improved properties of bone and cartilage tissue from 3D inkjet-bioprinted human mesenchymal stem cells by simultaneous deposition and photocrosslinking in PEG-GelMA. Biotechnol Lett. 2015 Nov;37(11):2349-55.
  36. Cui X, Lu YW, †Lee V, †Kim D, †Dorsey T, Wang Q, Lee Y, Vincent P, Schwarz J, Dai G.  Venous Endothelial Marker COUP-TFII Regulates the Distinct Pathologic Potentials of Adult Arteries and Veins. Scientific Reports, 2015 Nov 5;5:16193.
  37. Lee V, Dai G, 3-D Bioprinting and Tissue Fabrication: Prospects for Drug Discovery and Regenerative Medicine. Advanced Health Care Technologies, 2015, 1:23-35
  38. Lee VK, Lanzi AM, Ngo H, Yoo SS, Vincent PA, Dai G. Generation of Multi-Scale Vascular Network System within 3D Hydrogel using 3D Bio-Printing Technology. Cell Mol Bioeng. 2014 Sep;7(3):460-472. *2014 BMES Outstanding Contribution.
  39. Lee VK, Kim DY, Ngo H, Lee Y, Seo L, Yoo SS, Vincent PA, Dai G. Creating Perfused Functional Vascular Channels Using 3D Bio-Printing Technology. Biomaterials. 2014 Sep;35(28):8092-102. *Top 10 most downloaded material sciences paper since January 2014; Source: ScienceDirect.
  40. Lee V, Singh G, Trasatti JP, Bjornsson C, Xu X, Tran TN, Yoo SS, Karande P and Dai G. Design and fabrication of human skin by three-dimensional bioprinting. Tissue Eng Part C Methods. 2014 Jun;20(6):473-84.
  41. Song W, An D, Kao DI, Lu YC, Dai G, Chen S and Ma M. Nanofibrous microposts and microwells of controlled shapes and their hybridization with hydrogels for cell encapsulation. ACS Appl Mater Interfaces. 2014 May 28;6(10):7038-44.
  42. Cui X, Gao G, Yonezawa T, Dai G. Human Cartilage Tissue Fabrication Using Three-dimensional Inkjet Printing Technology. J Vis Exp. 2014 Jun 10;(88).
  43. Gao G, Schilling AF, Yonezawa T, Wang J, Dai G, †Cui X. Bioactive nanoparticles stimulate bone tissue formation in bioprinted three-dimensional scaffold and human mesenchymal stem cells. Biotechnol J. 2014 Oct;9(10):1304-11.
  44. Ozturk MS, †Lee VK, Zhao L, Dai G and Intes X. Mesoscopic fluorescence molecular tomography of reporter genes in bioprinted thick tissue. J Biomed Opt. 2013 Oct;18(10):100501.
  45. Pandit V, Zuidema JM, Venuto KN, Macione J, Dai G, Gilbert RJ and Kotha SP. Evaluation of multifunctional polysaccharide hydrogels with varying stiffness for bone tissue engineering. Tissue Eng Part A. 2013 Nov;19(21-22):2452-63.
  46. Zhao L, †Lee VK, Yoo SS, Intes X and Dai G. The integration of 3-D cell printing and mesoscopic fluorescence molecular tomography of vascular constructs within thick hydrogel scaffolds. Biomaterials. 2012 Jul;33(21):5325-32.
  47. Janet JL, Dai G, Chrisey DB. In search of the holy grail: engineering the stem cell niche. European Pharmaceutical Review, 2011, Issue 16, Volume 2.
  48. Lee YB, Polio S, Lee W, Dai G, Menon L, Carroll RS and Yoo SS. Bio-printing of collagen and VEGF-releasing fibrin gel scaffolds for neural stem cell culture. Exp Neurol. 2010 Jun;223(2):645-52.
  49. Dai G, Vaughn S, Zhang Y, Wang ET, Garcia-Cardena G and Gimbrone MA. Biomechanical forces in atherosclerosis-resistant vascular regions regulate endothelial redox balance via phosphoinositol 3-kinase/Akt-dependent activation of Nrf2. Circ Res. 2007 Sep 28;101(7):723-33.
  50. Parmar KM, Larman HB, Dai G, Zhang Y, Wang ET, Moorthy SN, Kratz JR, Lin Z, Jain MK, Gimbrone MA and García-Cardeña G. Integration of flow-dependent endothelial phenotypes by Kruppel-like factor 2. J Clin Invest. 2006 Jan;116(1):49-58.
  51. Parmar KM, Nambudiri V, Dai G, Larman HB, Gimbrone MA and García-Cardeña G. Statins exert endothelial atheroprotective effects via the KLF2 transcription factor. J Biol Chem. 2005 Jul 22;280(29):26714-9.
  52. Dai G, Kaazempur-Mofrad MR, Natarajan S, Zhang Y, Vaughn S, Blackman BR, Kamm RD, Garcia-Cardena G and Gimbrone MA, Jr. Distinct endothelial phenotypes evoked by arterial waveforms derived from atherosclerosis-susceptible and -resistant regions of human vasculature. Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14871-6.
  53. Tschumperlin DJ, Dai G, Maly IV, Kikuchi T, Laiho LH, McVittie AK, Haley KJ, Lilly CM, So PT, Lauffenburger DA, Kamm RD and Drazen JM. Mechanotransduction through growth-factor shedding into the extracellular space. Nature. 2004 May 6;429(6987):83-6.

Patents

Biodegradable Elastic Hydrogels for Bioprinting, filed April 4, 2018, U.S. Patent Application No. 17/044,527

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